Human uPAR enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 8.79 pg/ml (50 μl) |
| Standard Curve Range | 27.43~20000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0148 | 0.0166 | 0.0157 | |
| 27.43 | 0.0262 | 0.0238 | 0.0250 | 0.0093 |
| 82.30 | 0.0453 | 0.0491 | 0.0472 | 0.0315 |
| 246.91 | 0.1061 | 0.1035 | 0.1048 | 0.0891 |
| 740.74 | 0.2701 | 0.2723 | 0.2712 | 0.2555 |
| 2222.22 | 0.7935 | 0.7777 | 0.7856 | 0.7699 |
| 6666.67 | 2.0410 | 2.0280 | 2.0345 | 2.0188 |
| 20000.00 | 3.9180 | 3.9010 | 3.9095 | 3.8938 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 370.1 | 1850.1 | 6148.8 | 356.9 | 1784.4 | 6135.0 |
| Standard Deviation | 10.2 | 87.9 | 204.1 | 18.4 | 88.2 | 389.3 |
| Coefficient of Variation(%) | 2.8 | 4.8 | 3.3 | 5.2 | 4.9 | 6.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human uPAR into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 103% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1694.27-8807.19 | 100 | 3895.95 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of uPAR in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: uPAR
The urokinase-type Plasminogen Activator Receptor (uPAR) is a GPI-anchored protein that binds to both the inactive and active forms of uPA. This interaction serves to localize and enhance the proteolytic activity of uPA, leading to increased conversion of Plasminogen to Plasmin. uPA also triggers uPAR-mediated signal transduction, activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can additionally regulate cell adhesion by direct binding to Vitronectin and preventing Integrins from binding to their ligands.
