Human tPA enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.03 ng/ml (50 μl)；0.10 ng/ml (10 μl)； |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0058 | 0.0064 | 0.0061 | |
| 0.14 | 0.0107 | 0.0096 | 0.0102 | 0.0041 |
| 0.41 | 0.0195 | 0.0181 | 0.0188 | 0.0127 |
| 1.23 | 0.0457 | 0.0450 | 0.0454 | 0.0393 |
| 3.70 | 0.1406 | 0.1261 | 0.1334 | 0.1273 |
| 11.11 | 0.4191 | 0.3776 | 0.3984 | 0.3923 |
| 33.33 | 1.1900 | 1.1700 | 1.1800 | 1.1739 |
| 100.00 | 2.8370 | 2.8780 | 2.8575 | 2.8514 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 1.8 | 9.0 | 29.2 | 1.8 | 9.4 | 31.7 |
| Standard Deviation | 0.1 | 0.4 | 1.5 | 0.1 | 0.4 | 1.8 |
| Coefficient of Variation(%) | 5.7 | 4.6 | 5.1 | 5.2 | 3.9 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human tPA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 115% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.24-20.65 | 100 | 9.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of tPA in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: PAI-1/Serpin E1
PLAT, also known as tissue-type plasminogen activator (tPA), is a secreted serine protease synthesized by endothelial cells (1). The partially active single chain can be further processed to full activity by plasmin, tissue kallikrein or Factor Xa. Active PLAT converts plasminogen to plasmin, a fibrinolytic protease, by hydrolyzing an Arg-Val peptide bond in plasminogen. Unusually high levels of tPA activity can result in excessive bleeding, and low levels of tPA activity can result in thrombosis or embolism. Human PLAT contains 4 domains; the N-terminal fibronectin type-1 domain, an epidermal growth factor-like domain, two kringle domains and a serine protease catalytic domain.
