Human PDGF-AA enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.10 ng/ml (50 μl)；0.17 ng/ml (10 μl) |
| Standard Curve Range | 0.31~20 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.2311 | 0.2403 | 0.2357 | |
| 0.31 | 0.2942 | 0.3011 | 0.2977 | 0.0619 |
| 0.63 | 0.3271 | 0.3783 | 0.3527 | 0.1170 |
| 1.25 | 0.4328 | 0.4453 | 0.4391 | 0.2034 |
| 2.50 | 0.6121 | 0.6172 | 0.6147 | 0.3790 |
| 5.00 | 1.0300 | 1.0830 | 1.0565 | 0.8208 |
| 10.00 | 1.8060 | 1.8730 | 1.8395 | 1.6038 |
| 20.00 | 3.0540 | 3.0790 | 3.0665 | 2.8308 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 0.41 | 2.16 | 6.48 | 0.50 | 2.35 | 6.85 |
| Standard Deviation | 0.03 | 0.11 | 0.29 | 0.03 | 0.15 | 0.35 |
| Coefficient of Variation(%) | 6.7 | 5.2 | 4.5 | 7.0 | 6.2 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human PDGF-AA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 98% to 112% with an overall mean recovery of 106%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 21.10-57.89 | 100 | 33.58 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of PDGF-AA in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: PDGF-AA
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
