Human/Mouse BDNF enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.05 pg/ml (50 μl)；2.53 pg/ml (10 μl) |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Full-Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0195 | 0.0195 | 0.0195 | |
| 1.37 | 0.0241 | 0.0238 | 0.0240 | 0.0045 |
| 4.12 | 0.0410 | 0.0387 | 0.0399 | 0.0204 |
| 12.35 | 0.0961 | 0.0886 | 0.0924 | 0.0729 |
| 37.04 | 0.2670 | 0.2588 | 0.2629 | 0.2434 |
| 111.11 | 0.7864 | 0.7770 | 0.7817 | 0.7622 |
| 333.33 | 1.9080 | 1.9160 | 1.9120 | 1.8925 |
| 1000.00 | 3.3430 | 3.3080 | 3.3255 | 3.3060 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 26.4 | 132.7 | 428.3 | 26.0 | 132.0 | 417.2 |
| Standard Deviation | 0.9 | 5.5 | 28.5 | 0.8 | 5.2 | 22.7 |
| Coefficient of Variation(%) | 3.4 | 4.1 | 6.7 | 3.1 | 4.0 | 5.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recover y was evaluated by spiking 3 levels of human BDNF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 118% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.32-21.28 | 100 | 16.8 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of BDNF in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
