Human IL-7 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.62 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0139 | 0.0144 | 0.0142 | |
| 2.74 | 0.0221 | 0.0202 | 0.0212 | 0.007 |
| 8.23 | 0.0347 | 0.0347 | 0.0347 | 0.021 |
| 24.69 | 0.0817 | 0.0730 | 0.0774 | 0.063 |
| 74.07 | 0.2122 | 0.1976 | 0.2049 | 0.191 |
| 222.22 | 0.6447 | 0.5979 | 0.6213 | 0.607 |
| 666.67 | 1.6810 | 1.5880 | 1.6345 | 1.620 |
| 2000.00 | 3.7070 | 3.5910 | 3.6490 | 3.635 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 40.2 | 198.2 | 606.4 | 41.1 | 221.2 | 654.7 |
| Standard Deviation | 2.1 | 11.2 | 19.1 | 2.4 | 10.6 | 21.7 |
| Coefficient of Variation(%) | 5.3 | 5.6 | 3.1 | 5.9 | 4.8 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-7 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 96% with an overall mean recovery of 88%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-30.80 | 25 | 10.94 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-8 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-7
IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.
