Human IL-2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.67 pg/ml (50 μl) ；3.08 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0112 | 0.0111 | 0.0112 | |
| 6.86 | 0.0212 | 0.0173 | 0.0193 | 0.0085 |
| 20.58 | 0.0301 | 0.0282 | 0.0292 | 0.0175 |
| 61.73 | 0.0671 | 0.0623 | 0.0647 | 0.0530 |
| 185.19 | 0.2142 | 0.1983 | 0.2063 | 0.1955 |
| 555.56 | 0.8721 | 0.7723 | 0.8222 | 0.8105 |
| 1666.67 | 2.4952 | 2.4073 | 2.4513 | 2.4401 |
| 5000.00 | 4.2972 | 4.3173 | 4.3073 | 4.2961 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 92.1 | 407.5 | 1540.9 | 102.1 | 502.1 | 1990.9 |
| Standard Deviation | 4.7 | 23.4 | 86.4 | 6.0 | 29.1 | 119.09 |
| Coefficient of Variation(%) | 5.1 | 5.7 | 5.6 | 5.9 | 5.8 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 115% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d. | 0 | 0 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-2
Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a 15-18 kDa variably glycosylated alpha -helical polypeptide that is a member of the Common gamma Chain ( gamma c) cytokine family. It exists as a monomer and has a notably short half-life (< 30 minutes). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region. The mature region is alpha -helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity. Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma δ T cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells.
The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain. IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gamma c to form the quaternary signaling complex. In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gamma c also serves as a signaling receptor for IL-4, -7, -9, -15, and -21.
In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells.
