Human IL-15 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.14 pg/ml (50 μl)；0.79 pg/ml (10 μl) |
| Standard Curve Range | 0.69~500 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0090 | 0.0090 | 0.0090 | |
| 0.69 | 0.0201 | 0.0190 | 0.0196 | 0.0106 |
| 2.06 | 0.0460 | 0.0419 | 0.0440 | 0.0350 |
| 6.17 | 0.1111 | 0.1045 | 0.1078 | 0.0988 |
| 18.52 | 0.3220 | 0.2937 | 0.3079 | 0.2989 |
| 55.56 | 0.8509 | 0.8028 | 0.8269 | 0.8179 |
| 166.67 | 2.0501 | 1.8177 | 1.9339 | 1.9249 |
| 500.00 | 3.5230 | 3.4139 | 3.4685 | 3.4595 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 10.0 | 38.9 | 102.9 | 16.1 | 70.4 | 166.2 |
| Standard Deviation | 0.5 | 1.8 | 5.0 | 0.9 | 4.7 | 8.5 |
| Coefficient of Variation(%) | 4.8 | 4.7 | 4.9 | 5.8 | 6.7 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-15 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 142% to 189% with an overall mean recovery of 173%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.17-4.91 | 100 | 2.5 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-8 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-15
IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.
