Human IL-13 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 38.56 pg/ml (50 μl)；179.32 pg/ml (10 μl) |
| Standard Curve Range | 312.5~20000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0170 | 0.0154 | 0.0162 | |
| 312.50 | 0.0408 | 0.0397 | 0.0403 | 0.0241 |
| 625.00 | 0.0763 | 0.0760 | 0.0762 | 0.0600 |
| 1250.00 | 0.1793 | 0.1847 | 0.1820 | 0.1658 |
| 2500.00 | 0.4547 | 0.4510 | 0.4529 | 0.4367 |
| 5000.00 | 1.1861 | 1.1805 | 1.1833 | 1.1671 |
| 10000.00 | 2.5982 | 2.6327 | 2.6155 | 2.5993 |
| 20000.00 | 4.0628 | 4.1605 | 4.1117 | 4.0955 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 454.6 | 2142.1 | 6330.9 | 450.7 | 2260.8 | 6366.3 |
| Standard Deviation | 21.4 | 44.0 | 120.2 | 17.9 | 120.8 | 174.4 |
| Coefficient of Variation(%) | 4.7 | 2.1 | 1.9 | 4.0 | 5.3 | 2.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-13 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 107% to 114% with an overall mean recovery of 111%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-16.72 | 16.7 | 12.91 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-13 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-13
IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.
