Human IL-12 / IL-23 p40 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 13.59 pg/ml (50 μl)；37.7 pg/ml (10 μl) |
| Standard Curve Range | 40.96~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0472 | 0.0463 | 0.0468 | |
| 40.96 | 0.0589 | 0.0559 | 0.0574 | 0.0107 |
| 102.40 | 0.0741 | 0.0723 | 0.0732 | 0.0265 |
| 256.00 | 0.1179 | 0.1121 | 0.1150 | 0.0683 |
| 640.00 | 0.2334 | 0.2185 | 0.2260 | 0.1792 |
| 1600.00 | 0.5159 | 0.5153 | 0.5156 | 0.4689 |
| 4000.00 | 1.1800 | 1.1920 | 1.1860 | 1.1393 |
| 10000.00 | 2.4190 | 2.4810 | 2.4500 | 2.4033 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 215.15 | 1000.17 | 3317.32 | 222.76 | 1042.31 | 3324.53 |
| Standard Deviation | 8.59 | 41.69 | 201.12 | 11.97 | 22.87 | 199.61 |
| Coefficient of Variation(%) | 4.0 | 4.2 | 6.1 | 5.4 | 2.2 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-12/IL-23 p40 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 129% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-50.58 | 56.3 | 22.52 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-12 / IL-23 p40
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
