Human ICAM-1 / CD54 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 4.30 pg/ml(50 μl)；34.71 pg/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0128 | 0.0150 | 0.0139 | |
| 13.72 | 0.0217 | 0.0241 | 0.0229 | 0.0090 |
| 41.15 | 0.0372 | 0.0347 | 0.0360 | 0.0221 |
| 123.46 | 0.0720 | 0.0770 | 0.0745 | 0.0606 |
| 370.37 | 0.1703 | 0.1777 | 0.1740 | 0.1601 |
| 1111.11 | 0.4687 | 0.4474 | 0.4581 | 0.4442 |
| 3333.33 | 1.2790 | 1.2190 | 1.2490 | 1.2351 |
| 10000.00 | 2.8090 | 2.9300 | 2.8695 | 2.8556 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 234.1 | 1082.2 | 3166.5 | 213.4 | 1189.1 | 3504.5 |
| Standard Deviation | 19.8 | 43.9 | 70.1 | 17.3 | 46.1 | 211.0 |
| Coefficient of Variation(%) | 8.4 | 4.1 | 2.2 | 8.1 | 3.9 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ICAM-1/CD54 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 120% with an overall mean recovery of 118%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 46.75-110.67 | 100.0 | 88.85 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ICAM-1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: ICAM-1 / CD54
Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.
Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.
