Human ICAM-1 / CD54 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.49 pg/ml (50 μl)；6.36 pg/ml (10 μl) |
| Standard Curve Range | 41.15~30000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0073 | 0.0073 | 0.0073 | |
| 41.15 | 0.0092 | 0.0095 | 0.0094 | 0.0021 |
| 123.46 | 0.0167 | 0.0149 | 0.0158 | 0.0085 |
| 370.37 | 0.0389 | 0.0368 | 0.0379 | 0.0306 |
| 1111.11 | 0.1437 | 0.1413 | 0.1425 | 0.1352 |
| 3333.33 | 0.6950 | 0.6774 | 0.6862 | 0.6789 |
| 10000.00 | 2.1350 | 2.0700 | 2.1025 | 2.0952 |
| 30000.00 | 3.7320 | 3.6030 | 3.6675 | 3.6602 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 750.8 | 2955.8 | 8132.1 | 699.4 | 3236.1 | 8913.5 |
| Standard Deviation | 38.6 | 163.5 | 460.8 | 29.1 | 188.7 | 535.4 |
| Coefficient of Variation(%) | 5.1 | 5.5 | 5.7 | 4.2 | 5.8 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ICAM-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 103% with an overall mean recovery of 99%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 111.33-174.86 | 100 | 140.01 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ICAM-1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: ICAM-1 / CD54
Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.
Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.
