Human CXCL10/IP-10 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.33 pg/ml (50 μl)；1.14 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0119 | 0.0131 | 0.0125 | |
| 2.74 | 0.0165 | 0.0173 | 0.0169 | 0.0044 |
| 8.23 | 0.0291 | 0.0303 | 0.0297 | 0.0172 |
| 24.69 | 0.0657 | 0.0682 | 0.0670 | 0.0545 |
| 74.07 | 0.1921 | 0.1924 | 0.1923 | 0.1798 |
| 222.22 | 0.6346 | 0.6597 | 0.6472 | 0.6347 |
| 666.67 | 1.8938 | 1.9571 | 1.9255 | 1.9130 |
| 2000.00 | 3.6549 | 3.8681 | 3.7615 | 3.7490 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 38..3 | 189.7 | 714.0 | 46.6 | 195.6 | 731.2 |
| Standard Deviation | 1.6 | 11.9 | 33.3 | 2.7 | 12.7 | 41.3 |
| Coefficient of Variation(%) | 4.2 | 6.3 | 4.7 | 5.7 | 6.5 | 5.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL10 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 109% with an overall mean recovery of 95%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.06-52.70 | 100 | 16.04 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL10 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CXCL10/IP-10
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
