Human CXCL1/GRO alpha enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 14.89 pg/ml (50 μl)；32.67 pg/ml (10 μl) |
| Standard Curve Range | 20.48~5000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0137 | 0.0129 | 0.0133 | |
| 20.48 | 0.0170 | 0.0161 | 0.0166 | 0.0033 |
| 51.20 | 0.0261 | 0.0234 | 0.0248 | 0.0115 |
| 128.00 | 0.0446 | 0.0443 | 0.0445 | 0.0312 |
| 320.00 | 0.1492 | 0.1474 | 0.1483 | 0.1350 |
| 800.00 | 0.4857 | 0.5021 | 0.4939 | 0.4806 |
| 2000.00 | 1.2570 | 1.2630 | 1.2600 | 1.2467 |
| 5000.00 | 2.2760 | 2.2510 | 2.2635 | 2.2502 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 119.3 | 509.0 | 1409.9 | 110.0 | 510.1 | 1498.1 |
| Standard Deviation | 9.4 | 39.3 | 109.4 | 5.4 | 27.0 | 100.4 |
| Coefficient of Variation(%) | 7.9 | 7.7 | 7.8 | 4.9 | 5.3 | 6.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 72% to 125% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 77.28-616.13 | 100 | 358.62 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CXCL1/GRO alpha
CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.
