Mouse CXCL2/MIP-2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 10.2 pg/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0424 | 0.0439 | 0.0432 | |
| 13.72 | 0.0582 | 0.0608 | 0.0595 | 0.0164 |
| 41.15 | 0.0951 | 0.0965 | 0.0958 | 0.0527 |
| 123.46 | 0.2012 | 0.2071 | 0.2042 | 0.1610 |
| 370.37 | 0.5519 | 0.5019 | 0.5269 | 0.4838 |
| 1111.11 | 1.3550 | 1.2850 | 1.3200 | 1.2769 |
| 3333.33 | 2.8640 | 2.7400 | 2.8020 | 2.7589 |
| 10000.00 | 4.0796 | 4.0140 | 4.0468 | 4.0037 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 160.2 | 1021.4 | 3095.3 | 153.9 | 1034.9 | 3264.4 |
| Standard Deviation | 8.9 | 64.2 | 148.8 | 9.0 | 55.2 | 180.8 |
| Coefficient of Variation(%) | 5.6 | 6.3 | 4.8 | 5.9 | 5.3 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL2/MIP-2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 114% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-306.07 | 93.3 | 84.02 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL2/MIP-2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CXCL2/MIP-2
CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.
