Mouse CXCL2/MIP-2 enzyme-linked immunoassay kit

CAT: EM0018 Datasheet
Specification 96 Test
Sensitivity 10.2 pg/ml (10 μl)
Standard Curve Range 13.72~10000 pg/ml
Standard Curve Gradient 7 Points/3 Folds
Number of Incubations 2
Sample Volume 10 μl
Type Fully Ready-to-Use
Operation Duration 120min
pg/ml O.D. Average Corrected
0.00 0.0130 0.0122 0.0126
13.72 0.0151 0.0142 0.0147 0.0021
41.15 0.0210 0.0210 0.0210 0.0084
123.46 0.0381 0.0411 0.0396 0.0270
370.37 0.1251 0.1312 0.1282 0.1156
1111.11 0.5023 0.5012 0.5018 0.4892
3333.33 1.7271 1.8113 1.7692 1.7566
10000.00 3.9910 4.0931 4.0421 4.0295

Precision

Intra-assay Precision Inter-assay Precision
Sample Number S1 S2 S3 S1 S2 S3
22 22 22 6 6 6
Average(pg/ml) 160.2 1021.4 3095.3 153.9 1034.9 3264.4
Standard Deviation 8.9 64.2 148.8 9.0 55.2 180.8
Coefficient of Variation(%) 5.6 6.3 4.8 5.9 5.3 5.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of mouse CXCL2/MIP-2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 114% with an overall mean recovery of 101%.

Sample Values

Sample Matrix Sample Evaluated Range (pg/ml) Detectable (%) Mean of Detectable (pg/ml)
Serum 30 n.d. 0 n.d.

Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL2/MIP-2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

Background: CXCL2/MIP-2

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.

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