Human CXCL5/ENA-78 ELISA Kit检测试剂盒
| 规格 | 96 Test |
|---|---|
| 灵敏度 | 0.29 pg/ml (50 μl)；2.99 pg/ml (10 μl)； |
| 标准曲线范围 | 20.48~5000 pg/ml |
| 标准曲线梯度 | 7点 |
| 孵育次数 | 2 |
| 可检测样本 | 可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。 |
| 加样体积 | 50 μl/10 μl |
| 类型 | 完全即用型 |
| 操作时长 | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0134 | 0.0136 | 0.0135 | |
| 20.48 | 0.0370 | 0.0376 | 0.0373 | 0.0238 |
| 51.20 | 0.0745 | 0.0774 | 0.0760 | 0.0625 |
| 128.00 | 0.1724 | 0.1706 | 0.1715 | 0.1580 |
| 320.00 | 0.4228 | 0.4138 | 0.4183 | 0.4048 |
| 800.00 | 1.0020 | 1.0140 | 1.0080 | 0.9945 |
| 2000.00 | 2.2430 | 2.1530 | 2.1980 | 2.1845 |
| 5000.00 | 3.9350 | 3.8410 | 3.8880 | 3.8745 |
精度
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 135.3 | 659.5 | 1976.8 | 111.1 | 538.6 | 1723.8 |
| Standard Deviation | 8.7 | 37.6 | 102.5 | 4.3 | 17.4 | 78.9 |
| Coefficient of Variation(%) | 6.4 | 5.7 | 5.2 | 3.9 | 3.2 | 4.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
加标回收
健康人血清加入 3 个不同浓度水平的人 CXCL5/ENA-78 ，未加人 CXCL5/ENA-78 的血清作为本底，计算回收率。回收率的范围从 80 % 至 106 %，平均回收率为 89%。
样本值
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 1.93-7.91 | 100 | 3.83 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
产品数据表
背景: CXCL5/ENA-78
The protein encoded by this gene, Chemokine (C-X-C motif) ligand 5 (CXCL5), is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). It is observed that, CXCL5 also expresses in eosinophils, and can interact with the type II interferon IFN-α, thereby cause an inhibition. This chemokine stimulates the chemotaxis of neutrophils possesses angiogenic properties, and elicits these effects by interacting with the cell surface chemokine receptor CXCR2.
